Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology

Abstract Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly complex multipart from reusable basic parts a rapid, reliable automation-friendly way. Many such toolkits are available, with varying degrees compatibility, most which aimed at specific host organisms. Here, we present vector design allows simple modification by modular to assemble add functions secondary sites flanking the main insertion site (used for conventional cloning). Assembly all compatible PhytoBricks standard, vectors Standard European Vector Architecture (SEVA) as well BioBricks. We demonstrate that this facilitates construction tailored simplifies workflow generating libraries common elements. have made available collection 10 different microbial replication origins, copy number range, allowing chromosomal integration, selection commonly used parts. This expands range hosts can be easily modified acts toolkit facilitate generation required targeting further hosts.